Leica dm3000 pdf
While for SEM, small pieces of leaves were fixed in formaldehyde + acetic acid + alcohol (FAA) for 24 to 36 h, dehydrated in an ethanol series, critical point dried, mounted on stubs with self-adhesive double-sided carbon discs and sputter coated with gold. With the combination of easy adaptation to a user’s physical requirements and intelligent automation, the Leica DM3000 saves valuable time. After incubated with anti-E-cadherin or a-SMA (1:500) antibody at 4°C overnight, the sections were incubated with Alexa 488 Goat Anti-rabbit IgG and Alexa 594 Goat Anti-Rat IgG at room temperature for 1 hour. After cells were transfected for 48 h, they were digested with trypsin and washed with PBS. Cell viability was determined using a VICTOR X3 PerkinElmer 2030 Multilabel Plate Reader. The New, Automated Leica DM3000 Superior operating convenience meets ergonomic design – for even faster and more convenient work at the microscope.
The paraffin sections were then cut and finally stained with Delafield’s Hemato-xylin and Eosin staining (H&E staining). The images of the tissues were taken by a biological microscope (Leica DM3000, Germany). Quantification of muscle wall thickness and ganglion cell density was performed blind to the genotype with ImageJ. The Leica DM IL LED manual inverted microscope combined with the camera, for example, creates an affordable fluorescence package for cell transfection studies.
Our mission is to visualize your materials research in the very best light.
We super-posed a grid image with 36 points on histological fields per histological section. This delicate and important work requires excellent stereoscopic, 3D visualization combined with a long working distance to offer easy and conve-nient access for the tools of the operator. The cells were then treated with 5 μmol/L icaritin for 24, 48, or 72 h, and the control group was treated with the same volume of DMSO. Spindle Motor –40 Tools Auto Tool Changer –High Pressure Through the Spindle Coolant –6,000 RPM –Cat #50 Spindle Taper –Selca S3045 Controls –17,000 lbs. Values of villi height or crypt depth were the average of 3 measurements for each mouse. Leica Microscope User Manuals Download | ManualsLib The Leica OH3 is a high end surgical microscope for neurosurgery and spine operations. After they were permeabilized with 0.3% Triton X-100 and 0.1% sodium citrate, the cells were incubated with the TUNEL reaction mixture for 1 h at 37°C. Leica Biosystems hite Paper eries Ergonomics, Eficiency and Optical Performance in Clinical Microscopy - 3 - Figure 2: The optional Ergolift accessory underneath the microscope alters the distance from bench to eyepieces, which is particularly useful for tall users.
der bright-field microscopy (Leica DM3000) and the lines of arrested growth counted (LAGs) according to . Incubators Shown here is the Incubator S, which was designed for use with Petri dishes and glass slides. As a system microscope it is well suited not only for clinical microscopy, but for research microscopy applications as well. Leica Microsystems has many years of experience in developing inspection systems for the semiconductor industry. Background: The development of secondary brain injury via oxidative stress after traumatic brain injury (TBI) is a well known entity. After routine formalin‐fixed paraffin‐embedded specimen processing, 4 μm thick tissue sections were prepared and stained with haematoxylin and eosin, for histological evaluation of adipocyte morphology.
Subsequently, the internal circum-ference of each of the 10 sections from each site of the artery was determined using Scion image analyzer version 1.46. 1—Schematic of b phase grain growth by grooving of the a/b interphase (LAGB—low-angle grain boundary). Leica DM2000 Microscope: download pdf file: Overview Leica DM2000 with its sophisticated focus mechanism and high performance fluorescence has been developed for more complex tasks in pathology, cytology labs as well as in research applications. The microscope is designed for clinical laboratories, all biomedical routine and research applications, and is ideal for pathology applications where the user spends hours at a time at the micro-scope. This Leica DM microscope was a demonstration microscope that was used for a conference.
Luciferase Reporter Assay To explore whether NEAT1 could directly tar-get miR-376b-ap or miR-376b-3p could direct-ly target SULF1, the Luciferase reporter assay was applied. download pdf file: Overview The Standard in Inverted Digital Microscopy for materials research.
At the same time, MacScope soft-ware (Mitani Co., Fukuoka, Japan) was used to calculate the experimental CNV area at each light spot and calculate the mean value of each group. Plastic-coated surface provides high thermal conductivity rates & outstanding scratch resistance. Paraffin-embedded, formalin-fixed prostate tissues of patients and mice were immunostained for p62, Keap1 and Nrf2 protein using an immunohistochemical detection kit (GTVision) according to the manufacturer's protocol.
and visualized them under a compound microscope (Leica DM3000) at different magnifications ( 40–400). Our mission of “Advancing Cancer Diagnostics, Improving Lives” is at the heart of our corporate culture. A fibrosis index was derived by automated quantification of the proportion of tissue staining positive for collagen (red pixels), using ImageJ in at least 10 images per animal. x Switch from direct eye vision to camera exposure by pulling rod 14 (and photo A). The grid image comprised of all tooth sockets from the coronal limits adjacent to the gingival epithelium until the lower apical limit. Optically, it meets the same standards as the DM2500 and can also be used as a fluorescence microscope. The diameter of the microscope ﬁeld is known for each magniﬁcation and the camera provides a photo with a diagonal calibrated to the diameter of the microscope ﬁeld.
The sections were then observed microscopically (Leica DM3000).
The TUNEL assay was used to stain and quantify apoptotic cells in tissue sample slices according to the manufacturer's protocol. Subsequently, the cells were collected and re-suspended in 500 μl of 1× binding buffer. Our easy-to-use and consistently reliable offerings help improve workflow efficiency and diagnostic confidence. Leica’s wide range of heating and cooling inserts covers the needs of virtually every user. Pencil sketches were scanned at 300 dpi grayscale, and line drawings were created with Adobe Illustrator CS6 and a Wacom Bamboo Create tablet. Ten images at 400× magnification of randomly selected fields were taken on a Leica DM3000 microscope for quantitative histomorphometric analysis. Enzyme-Linked Immunosorbent Assay (ELISA) for VCAM-1 or ICAM-1 on Whole Cells Both ECs and HTR8/SVneo were grown up to conﬂuence in 96-well plates (105 cells/wells) and then treated with 15 g/mL ZnO for 48 h at 37 C in 5% v/v CO2 incubator. Cardiomyocyte stained cross-sectional areas of each group were measured and digitalized by Image-Pro Plus 6.0 software (Media Cybernetics, Silver Spring, MD, USA).
Intended Purpose of the Microscope The Leica DM1000 microscope, to which this user manual belongs, is designed for biological Caution! electron microscope, a single colony of each strain was inoculated in 10 ml YPD broth and . While doing my graduate LIDAR research we were often tasked with writing user guides for the equipment that we purchased to help others know how to use it.
Image analysis software (ImagePro Plus version 6.1, Media Cybernetics, Silver Spring, Maryland, USA) was used to measure the number of vessels per area (mm 2) and the mean diameter of ligniﬁ ed vessels ( D v ) near the cambial region in plants from differ- ent treatments. Bright, similar to daylight illumination at a low power consumption delivers reliable results while saving energy. Stage, Abbe, 4 Obj.Plan (4x,10x,40x,100x) 1 $1,310.00 20% $1,048.00 Educational * Power cord (13613900) MUST BE ORDERED SEPERATELY !!!! Fluorescence specimens are protected from unnecessary light, filter changes take place in the shortest time and new images can be acquired while the current one is transferred to PC. The Leica DM3000 microscopes for pathology, cytology, haematology and many other applications feature a motorized nosepiece, condenser head, automated light intensity adjustment, and optional foot pedal. Western blot analysis Western blotting was performed according to standard procedures. To detect the half-life time of GO nanosheets, the suspensions of GOsacrificed,nanosheets were intravenously injected into the mice (20formgdetectionkg-1).
histological sections by Leica DM3000 microscope at the Histology Department, Faculty of Veterinary Medicine, Benha University, Egypt. The photo-graphic plate was sensitive to blue light, but the human eye is more sensitive to yellow light. The spiral arteries undergo physiologic changes during pregnancy, and the failure of this process may lead to a spectrum of pregnancy disorders, including pre-eclampsia.
x To capture images, ask assistance to have the Leica DCF420C installed on the C-mount of the DM2500. Reepithelialization, granulation tissue formation, angiogenesis, and inflammatory cell infiltration were evaluated. Leica DM500 Microscope: download pdf file: The Leica DM500 microscope with “plug and play” capability is the ideal tool to make teaching entry-level college and university Life Science courses easy and fun for the instructor and the student.